EMD Millipore TLC Plate Glass Backed Silica 250μm 60 F254 2.5×7.5 cm

Description

Product Overview

The EMD Millipore TLC Plate is engineered for high‑performance thin layer chromatography (TLC) applications in research and quality‑control laboratories. Each plate features a robust glass backing that provides dimensional stability and resistance to warping during repeated handling and solvent exposure. The active layer consists of 250 μm thick silica gel (60 F254) impregnated with a fluorescent indicator that fluoresces under UV light, allowing rapid detection of separated compounds without the need for additional staining reagents. The plates are precision‑cut to a standard size of 2.5 × 7.5 cm, compatible with most TLC chambers and developing trays. Packaged in a box of 100 plates, the product offers a cost‑effective solution for high‑throughput screening, method development, and routine analytical workflows. Technical specifications include a package dimension of 6.5 × 3.2 × 1.1 inches and a net weight of 1.35 lb per box, ensuring easy storage and inventory management.

Usage

These TLC plates are suitable for a wide range of analytical scenarios, including organic synthesis monitoring, natural product isolation, pharmaceutical impurity profiling, and environmental sample analysis. The glass backing allows the plate to be placed directly on a heating block or a refrigerated surface without risk of deformation, making it ideal for temperature‑controlled separations. The 250 μm silica gel layer provides a balance between resolution and sample capacity, supporting both low‑volume spotting and larger sample loads. The F254 fluorescence indicator enables quick visualization of non‑UV‑active compounds after development, reducing turnaround time for results. Users can employ standard spotting techniques, such as capillary tubes or automated sample applicators, and develop the plates using common solvent systems ranging from non‑polar hexane‑based mixtures to polar aqueous‑organic blends. After development, plates are examined under a UV lamp (254 nm) to locate fluorescent spots, followed by optional staining for compounds that do not fluoresce.

Why Choose Us

Choosing the EMD Millipore TLC Plate means selecting a product backed by decades of expertise in chromatography media manufacturing. The glass backing is sourced from high‑quality borosilicate glass, ensuring chemical resistance and thermal stability across a broad temperature range. The silica gel layer is uniformly coated, providing consistent migration distances and reproducible Rf values across multiple plates and batches. Quality control procedures include rigorous testing for particle size distribution, moisture content, and fluorescence intensity, guaranteeing that each plate meets stringent performance criteria. In addition, the product is supported by a responsive technical support team that offers guidance on method development, troubleshooting, and best practices for TLC operation. Customers benefit from a reliable supply chain, fast shipping, and a clear return policy, reinforcing confidence in long‑term laboratory workflows.

Key Features

• Glass backing prevents warping, ensuring flat, stable plates for accurate separations.
• 250 μm thick silica gel layer delivers high resolution and sufficient sample capacity.
• F254 fluorescence indicator enables rapid spot detection under UV light without extra reagents.
• Standard 2.5 × 7.5 cm size fits most TLC chambers, simplifying method setup.
• Comes in a pack of 100 plates with consistent quality, backed by responsive customer support.

FAQ

What solvents can be used with these TLC plates?

The plates are compatible with a broad range of organic and aqueous solvents, including hexane, ethyl acetate, methanol, acetonitrile, and water‑based mixtures. Users should ensure that the solvent system does not exceed the chemical resistance limits of the glass backing.

How long can the plates be stored before use?

When kept in a cool, dry environment away from direct sunlight, the plates retain their performance for up to two years. It is recommended to store them in their original packaging to protect the silica layer from moisture.

Can the plates be reused after a separation?

These plates are intended for single‑use applications to maintain analytical integrity. Re‑using plates can lead to cross‑contamination and reduced resolution, so a fresh plate is advised for each experiment.

Is the F254 indicator safe for all types of samples?

The F254 indicator is generally safe for most organic compounds, but highly reactive or strongly acidic samples may affect fluorescence intensity. In such cases, alternative detection methods or post‑development staining can be employed.

What support is available if I encounter issues with the plates?

EMD Millipore provides technical assistance through email and phone support. Users can access troubleshooting guides, method development tips, and product documentation on the company website.