High Efficient H Type 5x10cm Glass Backed TLC Plate DONLAB

Description

Product Overview

The DONLAB High Efficient H model is a premium thin layer chromatography (TLC) plate designed for demanding laboratory applications. Constructed with a high‑quality glass backing, the plate offers superior rigidity and chemical resistance, ensuring that the silica layer remains intact during intensive analytical procedures. Each sheet measures 5x10cm and is supplied in packs of 40, providing ample material for routine assays and large‑scale projects. The silica particles are tightly controlled at 8 µm ±2 µm, delivering a uniform surface that promotes consistent capillary action and sharp spot development.

With a thickness ranging from 200 to 250 µm, the plate balances durability with optimal flow rates, allowing analysts to achieve rapid separations without sacrificing resolution. The High Efficient H formulation excludes calcined plaster and fluorescent indicators, reducing background noise and eliminating the need for special lighting conditions. This makes the plate especially suitable for colorless or weakly colored compounds where visual detection is critical.

Usage

These TLC plates are ideal for a broad spectrum of laboratory environments, including academic research labs, pharmaceutical quality control facilities, and industrial chemistry settings. The glass backing provides a stable platform for both manual spotting and automated sample application, ensuring that the plate can be handled with confidence during high‑throughput workflows. Users can employ the plates for the separation of organic compounds, natural products, pharmaceuticals, and polymers, making them a versatile tool for synthetic chemists, analytical scientists, and educators alike.

Because the plate does not contain fluorescent indicators, it can be used under standard laboratory lighting, simplifying the workflow and reducing the need for UV lamps. The three‑color separation system—yellow (BI‑methyl yellow), red (sultan red), and indigo—offers clear visual cues that help differentiate closely migrating spots, enhancing the accuracy of qualitative and semi‑quantitative analyses. The plates are compatible with a wide range of developing solvents, from non‑polar hexane mixtures to polar aqueous buffers, allowing users to tailor the mobile phase to the specific polarity of their analytes.

Why Choose Us

DONLAB has a long history of delivering reliable laboratory consumables, and the High Efficient H TLC plate is a testament to that commitment. Each batch undergoes rigorous quality control testing to verify particle size distribution, layer uniformity, and adhesion strength, ensuring that the plates meet the exacting standards required for reproducible chromatography. The glass backing is manufactured from optically clear, chemically inert glass, providing a non‑reactive surface that does not interfere with sample integrity.

Our plates are produced without calcined plaster, which can introduce variability and affect the mechanical stability of the silica layer. By eliminating this component, we achieve a smoother surface that reduces the risk of cracking or flaking during handling. Additionally, the absence of fluorescent indicators means that the plates are free from potential contaminants that could alter the chemical behavior of sensitive analytes.

Customers benefit from responsive technical support and a straightforward return policy, reinforcing our dedication to after‑sales service. Whether you are troubleshooting a challenging separation or need advice on optimal solvent systems, our experienced team is ready to assist.

Key Features

• Glass backed for exceptional rigidity and chemical resistance
• Uniform 8 µm silica particles ensure consistent capillary flow
• Three‑color separation system provides clear visual distinction of spots
• Thickness of 200‑250 µm balances durability with fast development times
• Dedicated technical support and hassle‑free return policy for peace of mind

FAQ

What solvents can be used with this TLC plate?

The plate is compatible with a wide range of solvents, including non‑polar mixtures like hexane/ethyl acetate, polar solvents such as methanol/water, and buffered aqueous systems. Choose the solvent system based on the polarity of your analytes for optimal separation.

Can I use these plates for quantitative analysis?

Yes. The uniform particle size and consistent layer thickness provide reliable spot intensity, allowing for semi‑quantitative densitometry or image analysis when calibrated against known standards.

How should the plates be stored to maintain performance?

Store the plates in a cool, dry environment, preferably in the original sealed packaging. Avoid exposure to high humidity or direct sunlight, which can affect the silica layer and glass backing.

Is there a recommended method for visualizing non‑colored compounds?

For compounds that are not visible under normal lighting, you can apply post‑development staining reagents such as iodine vapor, ninhydrin, or specific colorimetric sprays. Because the plate lacks fluorescent indicators, these methods work without interference.

What is the difference between the H and High Efficient designations?

The “H” designation refers to the standard high‑performance silica layer, while “High Efficient” indicates a refined formulation that excludes calcined plaster and fluorescent indicators, delivering cleaner backgrounds and improved spot resolution.